细胞培养基PH如何调节

2025-04-05 17:33:59
推荐回答(2个)
回答1:

酸碱度常用pH表示,不同种类的食用菌各有其可以生长的pH范围。一般说来,木腐菌类适于在偏酸性的环境中生长,粪草腐生菌喜欢在偏碱性的基质中生长。如猴头菌适生范围为pH3~4,香菇、银耳为pH5~6,木耳为pH5.5~6.5。金针菇在pH4~8范围内均能生长,最适pH为6。粪草腐生菌中草菇为pH7.5~9,蘑菇为pH7.2~7.5。

为使所配制的培养基能在适宜的pH范围,应考虑:培养料的性质和配比,高温灭菌后pH会有所降低,食用菌在生长过程会产生某些有机酸使pH下降,有些培养料在堆制发酵过程中微生物的活动也会使培养料的pH改变。所以,培养料需高温灭菌的,应先将其pH略调高。此外,为了在培养过程中使培养基的pH能维持在适宜范围内,常在培养基中添加一定的缓冲剂。常用的磷酸二氢钾(KH2PO4)、磷酸氢二钾(K2HPO4)等无机盐,除能供给食用菌以磷、钾等矿质营养外,还能对pH的变化起缓冲作用。钙(Ca)不但能中和酸性,而且还有增强菌丝耐酸的作用。

测定培养基(培养料)pH的简易方法为:液体培养基可用广泛pH试纸直接测定。固体培养料,在加水拌匀后,可用力挤出料中所含水分,再用上述试纸测定。如果料较干,可加适量中性水,然后搅拌澄清,再用广泛pH试纸蘸其澄清液测定pH。实验室还可用pH计进行较精确测定。

回答2:

一般是用碳酸氢钠配成碱性~再用稀盐酸调成7.0左右~

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