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如何检测western抗体效价

2025-04-03 09:17:42

推荐回答（1个）

回答1：

行.
以下为例：供参考
用Western blot检测MUC1核心肽多克隆
抗体特异性：将纯化的8R．MUCPT蛋白(2孔)经
SDS—PAGE后,转移至硝酸纤维膜上；5％脱脂奶粉封
闭4℃ 12 h；将硝酸纤维膜剪成2条,每1个上样孔
为1条,分别置于一抗为1：500稀释的兔抗8R—
MUCF1r血清和1：500稀释的MUC1核心肽单克隆抗
体中,室温2 h；PBS洗5 min×3次；二抗分别为
1：500稀释的过氧化物酶标记的羊抗兔IgG和1：500
稀释的过氧化物酶标记的羊抗鼠I ,室温2 h；PBS
洗5 min×3次；DAB显色.

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